A set of dotted lines delineates a boundary 2 standard deviations away from the median. Sample quality check/histograms – FlowJo plots the median light intensity values for each parameter over time.This area is often helpful for troubleshooting data files when looking at transform or range issues. Parameters and Stains – this area can be helpful for getting a snapshot of what parameters exist, their associated staining protocols and relevant information.Compensation matrix – if there is compensation applied to your sample, the matrix values will be displayed here.Sample keyword list – this list will show all keywords associated with your the samples, as created in FlowJo or at the time of acquisition.Use the arrow buttons at the top right of the dialog to cycle through your samples. The Inspect dialog FCS keyword header values for the sample you selected in the Workspace (1), the compensation matrix associated with the file (2), parameter, stain and display information (3) and sample quality check histograms for each parameter (4). This dialog provides a quick and simple way to view sample properties and verify keyword or parameter values of interest.
You can access Sample Properties/Information by selecting a sample in the Workspace and choosing Inspect from the Populations band of the Workspace task in the Workspace, command-clicking/ right-clicking on a sample and choosing Inspect, or by double clicking on the circle badge to the left of a sample in the Workspace (dialog shown below). What keywords are associated with my FCS files? What does my compensation matrix look like? FlowJo v10 lets you quickly assess this information and more with the Inspect tool.
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